Short COI markers for freshwater macroinvertebrate metabarcoding

Short COI markers for freshwater macroinvertebrate metabarcoding

Species diversity of metazoan bulk samples can be rapidly assessed using Cytochrome c oxidase I (COI) metabarcoding. However, in cases where only degraded DNA is available, e.g. from poorly conserved museum specimens, eDNA filtered from water or gut content analyses, universal primer sets that amplify only a short COI fragment are advantageous. Using PrimerMiner, we optimised two universal prim...

Validation and Development of COI Metabarcoding Primers for Freshwater Macroinvertebrate Bioassessment

Corrigendum: Validation and Development of COI Metabarcoding Primers for Freshwater Macroinvertebrate Bioassessment

In the original article, the reference for Bista et al. (2017) was incorrectly written as “Carvalho, G. R., Walsh, K., Seymour, M., Hajibabaei, M., Lallias, D., Christmas, M., et al. (2017). Annual time-series analysis of aqueous eDNA reveals ecologically relevant dynamics of lake ecosystem biodiversity. Nat. Commun. 8, 1–11. doi: 10.1038/ncomms14087.” It should be “Bista, I., Carvalho, G. R., ...

Reliability of long vs short COI markers in identification of forensically important flies

AIM To compare the reliability of short and long cytochrome oxidase I gene fragment (COI) in identification of forensically important Diptera from Egypt and China. METHODS We analyzed 50 specimens belonging to 18 species. The two investigated markers were amplified by polymerase chain reaction (PCR) followed by direct sequencing. Nucleotide sequence divergences were calculated using the Kimur...

Random sampling causes the low reproducibility of rare eukaryotic OTUs in Illumina COI metabarcoding

DNA metabarcoding, the PCR-based profiling of natural communities, is becoming the method of choice for biodiversity monitoring because it circumvents some of the limitations inherent to traditional ecological surveys. However, potential sources of bias that can affect the reproducibility of this method remain to be quantified. The interpretation of differences in patterns of sequence abundance...